US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
- Location: 75212, Torpo, Northwest, United States
We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. If you adored this short article and you would certainly such as to get additional facts relating to n-acetylneuraminic acid factory kindly browse through the web site. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed.
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